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KMID : 0578319910010020193
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Molecules and Cells
1991 Volume.1 No. 2 p.193 ~ p.196
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Production and characterization of Monoclonal Antibodies Against Human Hepatoma Associated Antigen
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Lee Myung-Kyu
Hahn Kyung-Soo
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Abstract
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The spleen cells obtained after immunization of human hepatoma plasma membrane surface proteins were fused with SP2/0 myceloma cells. Among 192 wells, 153 positive growing wells (3.1 X 10´ fusion probability per myeloma cell) were obtained and the productivity of the antibody against the diethylnitrosamine-induced rat hepatoma specific antigen (65 kDa) was 15.61/o. After primary and secondary cloning, three positive hybridoma cell lines were established. These monoclonal antibodies show an immuno-reactivity against the 65-kDa proteins of human liver and diethylnitrosamine-induced rat hepatoma plasma membrane surface proteins.
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