|
KMID : 0578319910010020219
|
|
Molecules and Cells
1991 Volume.1 No. 2 p.219 ~ p.223
|
|
|
Modulation of cAMP Responsive Element Binding Proteins for Lactate Dehydrogenase A Gene Promoter during Regeneration Period of Rat Liver
|
|
Park, Sung-Ook
Bae, Suk-Chul/Lee, Mi-Young/Lee, Seung-Ki
|
|
|
Abstract
|
|
|
|
Hepatocyte nuclear proteins binding to the cAMP responsive element (CRE) of lactate dehydrogenase (LDH) A gene were detected during regeneration period of rat liver by Southwestern blotting method. Three species of hepatocyte nuclear CRE binding proteins with pI values of 8.7-9.2 were identified to have molecular masses of 31, 29, and 14 kDa. The CRE binding activities of the nuclear proteins were detected at 12 and 18 h after partial hepatectomy during which the concentrations of cytosolic cAMP and the transcriptional activities of LDH A gene were observed to increase. Additionally, the binding activities of the CRE binding proteins for the -´2P-labeled CRE probes were alleviated in a dose dependent manner by treatment of potato acid phosphatase. These results suggest that CRE-binding proteins can regulate the LDH A gene transcription by phosphorylation via protein kinase A-CAMP signal transduction pathway during regeneration period of partially hepatectomized rat liver.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|