|
KMID : 0578319910010030287
|
|
Molecules and Cells
1991 Volume.1 No. 3 p.287 ~ p.294
|
|
|
Isolation and characterization of cDNAs for Ribulose Bisphosphate Carboxylase Multigene Family in Rice (Oryza Sativum)
|
|
Moon, Eun Pyo
Lee, Kwang-Woong/Lee, Jon Seob/Choi, Yang Do/Kim, Han-Jip
|
|
|
Abstract
|
|
|
|
We have isolated 16 cDNA clones for the small subunit of ribulose-1,5-bisphosphate carboxylase (rbcS) in rice. Among these, the cDNA inserts from seven clones, S1, S3, S5, S7, S10, S12, and S13 were analysed by restriction enzymes. The cDNA insert in S10 includes a 525-bp coding region, and about 1-kb 5´ and 258-bp 3´ noncoding regions. Comparison with a corresponding genomic DNA sequence (rbcS gene) revealed that 92 by between the base position 145 and 236 from the initiation codon in genomic rbcS sequence is an intron and spliced out in the cDNA. The fact that all the cDNA clones were isolated from the cDNA library made of the mRNAs from the dark-grown etiolated leaves suggests that the rbcS gene is also expressed in the dark, presumably at the basal level. The restriction enzyme analysis revealed that some cDNA were different from others, and at least six kinds of cDNA were identified. The differences in sizes and restriction enzyme sites suggest that there are several different copies of rbcS genes. It is also possible that different mRNA´s are made from more than one transcription initiation sites of rbcS gene, which is suggested by the fact that the length difference in some cDNAs is mainly due to the difference in the 5´-noncoding region. We can not rule out possibility of differential splicing in 5´-noncoding region of some cDNAs, which also would result in the difference in the 5´-noncoding region.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|