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KMID : 0578319910010030325
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Molecules and Cells
1991 Volume.1 No. 3 p.325 ~ p.331
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Secretion of Streptokinase from Bacillus subtilis
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Kim, Sung Il
Lee, Se Yong/Byun, Si Myung
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Abstract
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To secret the streptokinase (SKC) from Bacillus subtilis, three different expression-secretion vectors were constructed. One of them was pB140SK-I which contained the full streptokinase gene (skc) of Streptococcus equisimilis ATCC 9542 on pUB140 plasmid and the others were pB240SK-II and pB340SK-II which possessed the structural gene part of skc on secretion vectors pUBA240 and pUBS340, respectively. pUBA240 and pUBS340 are secretion vectors constructed with a-amylase promoter (amyRZ) and signal sequence. When these streptokinase secretion vectors were transferred into Bacillus subtilis LKS86, streptokinase was expressed and secreted into the culture broth. Although own promoter and signal sequence of the skc gene functioned normally in Bacillus subtilis, maximum amount of streptokinase secretion was obtained by LKS86(pB240SK-II) system.
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