|
KMID : 0578319910010030333
|
|
Molecules and Cells
1991 Volume.1 No. 3 p.333 ~ p.337
|
|
|
Swelling of Barley Protoplasts Modulated by Light/phytochrome and Ca2+ Ions
|
|
U, Zang-Kual
Song, Sung-Jun/Moon, Doo-khil/Song, Pill-Soon
|
|
|
Abstract
|
|
|
|
Effects of light/phytochrome, cAMP and Ca" modulators (EGTA and calimycin) on the swelling of protoplasts isolated from etiolated barley leaves have been studied as a possible model system for an understanding of the photo-signal transduction in plants. Protoplast swelling was induced by 2-min red-light irradiation, but not by far-red light, showing a typical phytochrome effect. Red light moderately increased the cellular cAMP level and enlarged the protoplast size, but its effect on the "Ca 21 ion uptake was not directly correlated with the swelling effect. Calimycin (Ca 2+ transporter across membrane) treatment increased both protoplast cAMP level and size, while the presence of EGTA decreased protoplast swelling, without affecting the cAMP level. Dibutyryl cAMP treatment directly induced protoplast swelling. Thus, the photoactivation of phytochrome by red light may lead to an increase in cytosolic Ca´+ concentration, which raises the cellular cAMP level and results in protoplast swelling.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|