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KMID : 0578319910010030339
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Molecules and Cells
1991 Volume.1 No. 3 p.339 ~ p.344
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Reefulation of Expression of a Potapo Proteinase Inhibitor I Gene in Transgenic Tobacco Plants
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Sohn, Hae won
yang, Yong Sung/Lee, Song Seob
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Abstract
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Potato proteinase inhibitor I (PI-1) genes are regulated developmentally in tubers and are wound-inducible in leaves. In order to investigate the regulatory mechanisms of a PI-1 gene, expression studies were carried out in tobacco plants with a chimeric gene constructed with the promoter of the PI-I gene, the bacterial chloramphenicol acetyl transferase (CAT) gene, and the terminator of a tomato inhibitor I gene. The chimeric PI-I-CAT gene was cloned into a binary vector derived from Ti-plasmid of Agrobacterium tumefaciens and was transferred into tobacco cells via Agrobacterium. Kanamycin-resistant transformed cells were selected and were regenerated to whole plants on various MS media containing 200 g/ml kanamycin sulfate. Expression of the PI-I-CAT gene was analyzed in transformed calluses and plants on various growth conditions. In transformed calluses, the PI-I-CAT gene was repressed by auxin but was induced in auxin-free medium. In leaves of transgenic plants, the gene was induced in a systemic manner by mechanical wounding.
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