|
KMID : 0578319910010030363
|
|
Molecules and Cells
1991 Volume.1 No. 3 p.363 ~ p.368
|
|
|
A Site-specific Deletion Mutant of Murin γ-Interferon Showing Anti0Viral Activity in Vitro
|
|
Lee Young-Ik
|
|
|
Abstract
|
|
|
|
Four amino acids were deleted in murine y-interferon gene using simple oligonucleotide directed mutagenesis in order to check whether the 4 amino acids were required for the anti-viral activity. Plasmid pYL-2 was constructed using expression vector pEV-vrf3 and the plasmid pMifko-1 which contains the whole coding sequence for the cDNA and 5´-and Y-noncoding sequences. Results demonstrated that deletion of the four amino acids from the amino-terminal region of the murine y-interferon still showed anti-viral activity in vitro.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|