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KMID : 0578319910010040465
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Molecules and Cells
1991 Volume.1 No. 4 p.465 ~ p.473
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Clonal Evolution in a Mixture of Six genetically Tagged Rat Hepatoma FTO2B Clones
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Lim, In Kyoung
Gerson, Stanton L./Hanson, Richard W.
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Abstract
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This paper describes selection in mixtures of clonal populations of FIF02B rat hepatoma cells marked with the neo and ada genes. Among the six clones, FTOPKA-S1 and S2/S3 became the prevalent clones either in the presence or absence of G418. Clones S4 and S5 disappeared during the clonal competition in G418 containing medium. However, clone
S4 became predominant in the mixture when G418 was removed from the medium. In constrast, clone S6 lost its resistance to G418 within 2 weeks when the clone was maintained without G418. Clone S6 disappeared after long incubation with G418. Clones S4 and S5 showed the lowest level of neo gene expression in the unstimulated state and a high response to the treatment of dexamethasone. Although the expression of the neo gene had effects on the selection of the predominant clones, individual clonal growth rates measured in the presence of G418 did not show any significant differences among the six clones. S1, S2/S3 and S6 were different from S4 and S5 in formation of monlayers and transformed foci. The former easily made compact monlayers and subsequently piled up in foci. The latter preferred to grow initially in piles with empty spaces between large foci. These characteristics may implicate the involvement of cellular adhesion molecules in determining the clonal selection among the cellular mixtures.
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