|
KMID : 0578319910010040483
|
|
Molecules and Cells
1991 Volume.1 No. 4 p.483 ~ p.489
|
|
|
The Effects of Increased Intracellular cANP on Dopamine Beta-Hydroxylase
|
|
Hwang, Onyou
Lee, Jae Dam/Lee, Chung Choo/Kim, Hye Jin
|
|
|
Abstract
|
|
|
|
Dopamine beta-hydroxylase (DBH) is the enzyme which is responsible for the synthesis of norepinephrine and epinephrine. Towards the understanding of DBH regulation at the molecular level, the effects of elevated intracellular cAMP on the steady state DBH mRNA level were examined. Forskolin, an activator of adenylate cyclase, increased the DBH mRNA 3-4-fold at 50 pM while 8-bromo-CAMP, a stable analogue of cAMP, resulted in a 7-8-fold increase at 100 M. These effects were dose-dependent, and were maximal at 6 h. The effects of forskolin and 8-bromo-CAMP were accompanied by increases in the intracellular contents of norepinephrine and epinephrine. The significance of the pre-sent results in relation to the existence of a putative cAMP-responsive element in the DBH gene promotor region and to the similar regulation seen with other catecholamine biosynthetic enzymes is discussed.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|