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KMID : 0578319920020010017
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Molecules and Cells
1992 Volume.2 No. 1 p.17 ~ p.22
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Genomic Cloning of the Extracellular Cellulase Gene From Xanthomonas campestris pv. Oryzae
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Yun, han Dea
Lim, Sun Tech/Chung, Min Hwa/Park, Yong Woo/Kim, Hee Kyu/Kang, Kyu Young
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Abstract
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Xanthomonas campestris pv. oryzae (Ishiyama) dye is a causal organism of bacterial leaf blight of rice (Oryzae sariva). It is known that the pathogens invade the host plant. The penetration of X. c. pv. oryzae into the plant requires the activity of cell wall hydrolyzing enzymes. Such an enzymatic process of infection was supported by smooth surfaces without trichomes upon inoculation and many penetrating bacterial cells through stomatal pore by scanning electron microscopy. The presence of pathogens in the mestome sheath of rice leaf was also confirmed by transmission electron microscopy. Furthermore, X. c. pv. oryzae exhibited the activities of extracellular cellulase and proteases as cell wall degrading enzymes by the agar diffusion method. To assess the contribution of the cell wall degrading enzymes to the pathogenicity of X c. pv. oryzae, we constructed a genomic library of X: c. pv. oryzae using the cosmid vector pLAFR3. Out of more than one thousand transductants, one extracellular cellulase and several protease clones were isolated. The clone, designated as pXC343, expresses cellulase activity in Escherichia coli background. This result was confirmed by mutagenesis with Tn5 to make a cel- mutant.
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