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KMID : 0578319920020020139
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Molecules and Cells
1992 Volume.2 No. 2 p.139 ~ p.143
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Characterization of the asp Mutations in Asparaginase Ⅱ-deficient yeast Strains
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Kim, Kyu-Won
Hong, Young-Soo/Lee, Su-Jae/Chung, Hae-Young
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Abstract
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We have studied the genomic structure of the ASPS gene in the wild type and asp mutant Saccharomyces cerevisiae strains. The asp2, asp3 and asp6 mutant strains did not synthesize asparaginase II under both nitrogen-starved and nitrogen-rich conditions. Southern blot analysis showed that the ASPS gene was completely deleted in the asp3 mutant strain. In contrast, the ASPS gene structure was essentially intact in the asp2 and asp6 mutant strains. Northern blot analysis indicated that the asp2 and asp6 mutants did not synthesize ASP3 mRNA in spite of the presence of an intact ASP3 gene. These results implied that the loss of asparaginase II was related to the extensive deletion of the structural gene in the asp3 mutant strain or probably some defects in the regulatory genes in the asp2 and asp6 mutant strains.
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KEYWORD
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