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KMID : 0578319920020020149
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Molecules and Cells
1992 Volume.2 No. 2 p.149 ~ p.154
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Role of Extracellular matrix Proteins on the Differentiation of the F9 Embryonal Carcinoma Cells
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Lee, Ho-young
Hur, Jyu-Chung/Kim, Kyu-Won
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Abstract
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In order to investigate the role of extracellular matrix (ECM) proteins in differentiation of F9 embryonal carcinoma (EC) cells, we have analyzed the expression of extracellular matrix components during the differentiation of F9 cells induced by retinoic acid (RA) and dibutyryl cyclic AMP (dbcAMP). The mRNA level of type IV collagen increased markedly and that of fibronectin decreased after the differentiation of F9 cells induced by RA and dbcAMP. The induced expression of laminin B1 in differentiated F9 cells did not change upon removal of RA and dbcAMP from culture medium. In addition, the effect of ECM components on the morphological behavior of F9 EC cells was examined by culturing F9 EC cells on the ECM-coated culture flask. When F9 EC cells were cultured on laminin- and laminin/type IV collagen-coated culture flasks, they rapidly formed a round cell morphology similar to the F9 cells differentiated by RA and dbcAMP. In contrast, F9 cells cultured on a fibronectin-coated flask grew packed colonies and exhibited a flattened appearance like the morphology of F9 EC cells. These results suggest that ECM proteins, laminin and type IV collagen, are important in the morphological change and differentiation of F9 EC cells. Moreover, employing immunofluorescence analysis, we proved that the distribution pattern of cytoskeletal protein, tubulin, markedly changed through the differentiation process of F9 cells. This result suggested that cytoskeletal proteins might interconnect with ECM molecules and these ECM-cytoskeletal linkages are most likely critical for morphological change of F9 cells.
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