|
KMID : 0578319920020020191
|
|
Molecules and Cells
1992 Volume.2 No. 2 p.191 ~ p.198
|
|
|
Characterization of a Potato Proteinase Inhibitor Ⅱ Gene that Is Expressed Constitutively in Transgenic Tobacco plants
|
|
Choi, Yeonhee
Kim, Joo Won/Lee, Jong Seob
|
|
|
Abstract
|
|
|
|
Proteinase inhibitor II genes are present as a small multigene family in the potato genome. We determined the nucleotide sequence of a potato inhibitor II (PI-II) gene on a 13.5-kb EcoRI fragment isolated from a potato genomic library. The inhibitor II (PI-II-13.5) gene codes for a pre-inhibitor II of 147 amino acids. The coding region is interrupted by an intervening sequnce of 113 bp, situated within codon 18 of the reading frame. The nucleotide sequence of its 5´ flanking region shares about 85% homology upto the 700-bp region from the transcription initiation site with other potato inhibitor II genes down-regulated by auxin and induced by wounding. In order to investigate the mode of expression of the PI-II-13.5 gene, a chimeric gene was constructed with the reporter chloramphenicol acetyltransferase (CAT) gene and transferred into tobacco cells through mediation of Agrobacterium tumefaciens. Kanamycin-resistant calluses were selected and were regenerated to transgenic plants. The CAT assay results showed that the expression of the chimeric PI-II-CAT gene was not affected by auxin in tobacco calluses and was not induced by mechanical wounding in transgenic plants. These suggest that the PI-II-13.5 gene may be a member of inhibitor genes that are expressed constitutively in the potato plant.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|