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KMID : 0578319920020020199
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Molecules and Cells
1992 Volume.2 No. 2 p.199 ~ p.205
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Different Transcription Termination Sequences of Gastin Genes
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Kim, Sun Jung
Kang, Joo Jyun/Kang, Yong Koo/Noh, Moon Jong/Lee, Kyung-Kwang/Yoo, Ook Joon
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Abstract
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We have isolated genomic clones that contain monkey, sheep and mouse gastrin genes. The terminator-like sequence of monkey gastrin gene for RNA polymerase II was similar to the previously shown human terminator [Sato, K, Ito, R., Baek, K.-H., and Agarwal, K (1986) Mol. Cell. Biol. 6, 1032-10431. To identify the termination element in mouse and sheep gastrin genes, we employed an in vivo transiently expressing system. The GT-repeat sequence at the 3´ flanking region of mouse gastrin gene, acted as a transcription terminator. When the element was deleted, the effect of termination disappeared perfectly. When the element was artificially inserted into the 3´ flanking region of sheep gastrin gene that had not shown any transcription termination, the effect of termination newly appeared. The GT-repeat element responsible for transcription termination functioned with orientation-dependent fashion. An adenine stretch (A)lo found at the 3´ flanking region of sheep gastrin gene, however, did not function as a terminator. Thus, mechanisms of transcription termination by RNA polymerase 11 appear to vary from species to species even for the same eukaryotic gene.
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