|
KMID : 0578319920020030257
|
|
Molecules and Cells
1992 Volume.2 No. 3 p.257 ~ p.263
|
|
|
Selection of Potential T Cell Epitopes by Determining the Bnding of Antigenic peptides to MHC on Antigen-presenting Cells
|
|
kwon, Hyoung Joo
lee, Hee Gu/Kim, Hee Sue/Choe, In-Seong/Chung, Tai-Wha/Kim, Seung-Ho/Chung, Jyeong-Soo
|
|
|
Abstract
|
|
|
Remodeling of extracellular matrix (ECM) is one of the key events in many developmental processes.
In the present study, a temporal profile of gelatinase activities in regenerating salamander limbs was examined zymographically.
In addition, the effect of retinoic acid (RA) on these enzyme activities was examined to relate the pattern-duplicating effect of RA in limb regenerates with gelatinase activities were detected, and these activities were at their maximum levels at the dediffe-rentiation stage.
Upon treatment with chelating agents EDTA and 1,10-phenanthroline, the enzyme activities were inhibited indicating that those enzymes are likely matrix metalloproteinases (MMPs).
Considering the molecular sizes and the decrease of molecular sizes by treatment with p-aminophenylmercuric acetate, an artificial activator of proMMP, some of the gelatinases expressed during limb regeneration are presumed to be MMP-2 and MMP-9.
In RA-treated regenerates, overall gelatinase activities increased, especially the MMP-2-like gelatinase activity which increased markedly.
These results suggest that MMP-2-like and MMP-9-like gelatinases play a role in ECM remodeling during regeneration, and that gelatinases are involved in the excessive dedifferentiation after RA treatment.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|