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KMID : 0578319920020030303
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Molecules and Cells
1992 Volume.2 No. 3 p.303 ~ p.308
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Genomic Structure and Nucleotide Sequence of the Polyhedrin Gene of hyphantria cunea Nuclear Polyhedrosis
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Lee, Hyung-Hoan
Min, Bon-Hong/Chung, Hye-Kyung/Lee, Keun-Kwang/Park, Jaeku/Cha, Soung-Chul/Seo, Neoung-Seon
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Abstract
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Using a differential display PCR, we identified a differentially expressed cDNA fragment which was detectable in retinoic acid (RA) treated F9 embryonal carcinoma (EC) cells but not in untreated F9 cells.
A homology search of the Gene Bank indicated that the cDNA fragment is part of the mouse homolog of the Drosophila Disabled (mDab2) gene.
Aggregate cultures of F9 EC cells grown in the presence of the RA differentiated into nonmalignant cells resembling the visceral endoderm of the mouse embryo.
Upon induction of endodermal differentiation with 10-7 MRA, the gene expression of mDab2 was increased gradually during the first 96 h.
Neither undifferentiated F9 cells, nor the undifferentiated aggregate cells without RA expressed mDab2.
Whole-mount in situhybridization and quantitative RT-PCR also showed that the temporal expression pattern of the mDab2 gene coincides with the initiation pattern of RA synthesis that occurs during mouse embryogenesis.
Also, two alternative splicing messages of mDab2 were detected in a tissue specific manner.
All the data indicate that mDab2 may play an important role in RA-induced signal transduction during mouse development.
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