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KMID : 0578319920020030353
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Molecules and Cells
1992 Volume.2 No. 3 p.353 ~ p.356
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A New Chromosomal Gene which Masks dsRNA Virus Cold Sensitivity in ski Saccharomyces cerevisiae
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Hwang Bo-The
Lee Hyun-Sook
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Abstract
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The yeast SKI genes, whose name was derived from the superkiller phenotype shown by mutant strains, inhibit cytopathology of L-A and M viruses through decreasing the copy number of the viron. The virus-infected skit mutant cells were transformed with a yeast genomic library and the healthy transformants were selected and analyzed. One of the transformants remained as a superkiller, although it did not have the cold-sensitive phenotype which is a typical ski- phenotype when the cell is infected with the virus. Introduction of the plasmid -DNA recovered from this transformant into the parent skit strain or into ski3 and ski6 mutants gives the same phenotype. These transformants also did not show abnormal budding pattern. Their viral dsRNA contents were not different from those of control cells. Thus, an apparently new chromosomal gene masks the cold sensitivity caused by viral infection in ski- background. The region responsible for the viral pathology masking phenotype was reduced from 10.5 kb to 2.5 kb.
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KEYWORD
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