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KMID : 0578319930030010007
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Molecules and Cells
1993 Volume.3 No. 1 p.7 ~ p.12
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Expression of a Chimeric antibody with Specificity for pre-S2 surface Antigen of hepatitis B Virus in Murine Myeloma Cell Line
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Jin, Byung Rae
Ryu, Chung Jeih/Park, Sung Sup/Hong, Hyo Jeong/Han, Moon Hi
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Abstract
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Hepatitis B virus (HBV) infection represents a worldwide health problem. For the prophylaxis and prevention of HBV infection, we have investigated on the production of mouse and human chimeric antibody which can bind to the pre-S2 surface antigen of HBV. The chimeric antibody genes were constructed by combining the mouse immunoglobulin heavy and light chain variable region (V and VL) genes with human rl and k constant region genes, respectively, which was then cloned into pSV2neo and pSV2hygro to yield expression plasmids pHS2-neo and pLS2-hygro, respectively. The plasmids were sequentially electroporated into murine non-Ig-producing hybridoma (SP2/0) cells, and transfectomas secreting chimeric antibody were identified by ELISA. The chimeric antibodies were purified and characterized by 101/o SDS-PAGE and western blot analysis. These results demonstrated that the transfectomas functionally express and secrete chimeric antibody which can bind to the pre-S2 surface antigen of HBV. The chimeric antibody may be useful to the therapy of HBV infection.
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