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KMID : 0578319930030010023
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Molecules and Cells
1993 Volume.3 No. 1 p.23 ~ p.26
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Cloning and Nucleotide Sequence of a cDNA clone Specifying Full Coding Exons of N-ras Gene from sprague-Dawley Rat
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Min Kyung-Rak
Kim Youngsoo
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Abstract
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A N-ras cDNA clone has been isolated and sequenced by application of a trascript-polymerase chain reaction (PCR) method. The cDNA sequence with 584 base pairs was selectively amplified from nanogram quantities of total RNA from Sprague-Dawley rat, and then directly cloned to M13mp18 and M13mpl9. Nucleotide sequencing has revealed that this cDNA clone has full coding exons encompassing initiation to termination codons of a N-ras gene which exhibits more than 90% sequence homology with N-ras proto-oncogenes of mouse and human. A deduced amino acid sequence of this rat cDNA exhibits only three and four amino acid differences when compared with the N-ras gene products of mouse and human. The amino acid differences have been found in positions 69, 168, and 175 of mouse N-ras and positions 69, 175, 184, and 188 of human N-ras. All of the substitution positions are located in hydrophilic domains which have been known as nonessential amino acids for biochemical properties of the mammalian N-ras gene products.
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KEYWORD
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