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KMID : 0578319930030010065
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Molecules and Cells
1993 Volume.3 No. 1 p.65 ~ p.69
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Accurate Detection of DNA single-straned Breaks by laser Scanning Microscopic Analysis of the Single Cell gel Assay is Subject to Several Parameters
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Fairbaim, Daryl W.
O´neill, Kim L./Meyers, C. Daniel/Standing, Michael D.
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Abstract
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DNA damage can lead to mutagenesis and carcinogenesis. The proper repair of various DNA modifications is recognized as important to the prevention of cellular transformation and cancer induction. DNA single-strand breaks that can potentially throw the cell into the complex multi-step process of carcinogenesis are normally repaired in human cells.
The accurate assessment of single-strand breaks in human cells can provide a valuable tool in research. We have shown that laser scanning microscopic analysis of the single cell gel (SCG) technique allows rapid, sensitive, and reproducible quantitation of single-stranded breaks in cellular DNA The SCG assay parameters can be altered to allow for accurate damage detection. In this paper we present some factors (including exposur concentration and time, electrophoresis time, cell type and the number of cells analyzed, and the amount of stain used) that influence the ability to accurately assess DNA damage using the single cell gel assay.
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