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KMID : 0578319930030010089
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Molecules and Cells
1993 Volume.3 No. 1 p.89 ~ p.93
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Differentiation Stage-Dependent Hepatitis B Virus Gene Expression in Human Liver Cells
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Rho, Hyune Mo
Kim, Seong Kee/Seo, Tae Suk/Choi, Cheol Yong/Jung, Gu Hung/Park, Geon Tae
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Abstract
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Hepatitis B virus (HBV) is characterized by its hepatotropism, unique genome structure and mode of replication. A series of cell lines and differentiation stage-different cells were used for the study of the presence of HBV receptors and HBV gene expression. The receptors for HBV were detected in human hepatoma cells but not in nonhepatoma cells, suggesting that the hepatotropism of HBV is due to the presence of HBV specific receptors. When the various differentiated human cell lines were transfected with cloned HBV DNA, the expression and secretion of HBV surface antigen (HBsAg) and core-related antigen (HBeAg) were related to the degree of liver cell differentiation. This observation proved that more differentiated liver cell produced more HBsAg and HBeAg. Six HBV-specific transcripts of 4.0, 3.5, 2.2, 21, 1.2 and 0.9 kb were also detected in highly differentiated HepG2 cells like in the case of HepG2-K8 cells producing the mutant HBV particles. HBV pregenomic promoter activity was stronger in the highly differentiated human hepatoma cell, HepG2, than in undifferentiated liver cell lines, SK-Hepl and HA22TNGH in CAT assay system. On the other hand, the non-liver cell lines, COSM6 and HeLa did not use the HBV pregenomic promoter. These results suggest that the synergistic action of differentiation stage-dependent transcription factors account for the propagation of HBV in highly differentiated human liver cells.
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