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KMID : 0578319930030020133
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Molecules and Cells
1993 Volume.3 No. 2 p.133 ~ p.136
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Cooperative Function of Upstream and Core Domains of the Yeast Ribosomal RNA Gene Promoter
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Rho, Jae Kyun
kwon, Hyuk ran/Reeder, Ronald H./Choe, Soo young
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Abstract
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We have previously reported that the yeast, Saccharomyces cerevisiae, ribosomal gene promoter contains at least two essential domains, an upstream domain located at the 5´ boundary near position -150 and a core domain around the site of transcription initiation at + 1, by in vitro analysis using whole cell yeast extracts. Here we show the activity of the promoter is highly sensitive to spacing changes between two domains, but the activity can be partially rescued when the spacing changes was either increased or decreased to 10 bp. The upstream or core domain DNA sequences alone could not compete with wild promoter in transcription initiation step, and the upstream and core domains are both necessary for transcription initiation complex formation. The upstream domain of the promoter, however, can be severed from the core promoter domain once the stable complex has been formed. These results suggest that the yeast ribosomal gene promoter has a critical requirement for binding of protein or protein complex to core and upstream domains to be located at precise positions on the face of the DNA helix.
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KEYWORD
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