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KMID : 0578319930030020143
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Molecules and Cells
1993 Volume.3 No. 2 p.143 ~ p.148
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The Role of the Extracellular Cellulase in Xanthomonas oryzae pv. Oryzae on Bacterial Leaf Blight
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Yun, Han Dae
Park, Young Woo/Lim, Sun Tech/Kim, Hee Kyu/Kang, Kyu Young
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Abstract
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A plasmid, designated pXC343, encoding an extracellular cellulase activity from Xanthomonas oryzae pv. oryzae (Ishiyama) was reported previously (Yun et ah, 1992). The cellular distribution of the cellulase activities of pXC343 and X. o. pv. oryzae was almost exclusively extracellular. Total cellulase activity expressed by the pXC343 was about 80% of that expressed by X. o. pv. oryzae K1 wild type. Tn5 mutagenesis of pXC343 revealed that the extracellular cellulase structural gene is located in about 5 kb KpnI fragment. Three cellulase-minus mutants of X o. pv. orynae constructed by Tn5 insertions into the chromosome of the wild type were as virulent as the wild type in pathogenicity tests on leaves of rice (Oryzae sadva L.). Further-more, results from the transmission electron microscopy (TEM) showed that bacterial leaf blight (BLB) symptoms used by the cellulase-minus mutants, X o. pv. oryzae KI::Tn5, were similar to those used by the wild type. These results suggest that the extracellular cellulase of X. o. pv, oryzae is minor factors for the pathogenesis of BLB, but might be involved in an early stage of disease development.
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