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KMID : 0578319930030020195
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Molecules and Cells
1993 Volume.3 No. 2 p.195 ~ p.202
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Full Genome Cloning and Nucleotide Sequence Analysis of Hepatitis C Virus from Sera of Chronic hepatitis Patients in Korea
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Cho, Young-Gyu
Woon, Jin-Won/Jang, Kyung-Lib/Kim, Chang-Min/Sung, Young-Chul
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Abstract
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The nucleotide sequence of a Korean type of hepatitis C virus (HCV-K) genome, consisting of 9,415 nucleotides, was determined by analysis of cDNA clones from sera of chronic hepatitis patients in Korea. The cDNA (HCV-K) has single large open reading frame of 9,030 nucleotides encoding a polyprotein of 3,010 amino acids, 5´ untranslated region (UTR) of 340 nucleotides, 3´ UTR of 45 uucleotides and additional T stretch of 43 to 87 nucleotides at the 3´ end. Comparison of HCV-K with the prototype of HCV cDNA (HCV-1) showed 78% homology at the nucleotide sequence level and 84% homology at the amino acid sequence level. On the other hand, HCV-K is closely related to the two Japanese isolates, HCV-J and HCVBK, with about 90% homology in the nucleotide and amino acid sequences. In contrast, it showed less than 70% sequence homology to the other Japanese isolates, HC-J6 and HC-J8. Therefbre, HCV-K can be classified into a distinct subtype closely related to Japanese isolates, HCV-J and HCV-BK.
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KEYWORD
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