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KMID : 0578319930030020203
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Molecules and Cells
1993 Volume.3 No. 2 p.203 ~ p.209
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Nucleotide Sequence of a Gene for ChlorophyⅡ a/b binding protein of Rice
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Lee, Min Gyu
kang, Yun Jung/Kim, Chung Ho/Choi, Jin Nam/Kim, Han-Jip/lee, Jong Seob/Lee, Kwang-Woong
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Abstract
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To study the regulatory mechanism of gene expression and the transport mechanism of chlorophyll a/b binding protein of photosystem H (CABII) into chloroplast, a cDNA clone and its genomic clone were isolated. cDNA was synthesized from mRNA isolated from fight-grown rice leaves, specifically amplified by polymerase chain reaction and was cloned directly into pUC18 vector. The nucleotide sequence of the cDNA clone, rcabll, Was determined. It contains 795 by of an open reading frame encoding a polypeptide of 265 amino acids, with 7 by of 5-noncoding and 186 by of Y-noncoding sequences. The genomic clone for rcabll gene, grcabll, was isolated. It contains 795-bp of coding sequence, which is identical to that of the cDNA clone rcabll, and its 5´- and 3´-flanking regions. The coding region of greabll is not interrupted by intervening sequence. Genomic Southern blot analysis suggested that the rcabll gene is a member of relatively small multigene family in the rice genome. A comparison of the deduced amino acid sequences with that of other cabll genes shows that the rcabll gene belongs to type I of cabll gene. Mature protein encoded by rcahH contains three hydro-phobic transmembrane domains and the transit peptide which has a theoretical propensity to form amphiphilic a-helical structure. Northern blot analysis revealed that mRNA size for the cloned cDNA is 1.1 kb long and its gene expression is regulated at transcriptional level in light-inducible manner. In the 5´ upstream regions of grmbll, conserved sequences in fight responsive genes are found such as the box III*, box n, G-box, 3AF1-binding site, an octamer repeat and phytochrome-responsive element. The function of these putative regulatory elements are discussed.
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KEYWORD
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