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KMID : 0578319930030020225
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Molecules and Cells
1993 Volume.3 No. 2 p.225 ~ p.228
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Bone Morphpgenetic Protein and Transforming Growth Factor-β Increase DNA and Protein Synthesis in Cultured Osteoblast Cells
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Kim, Geum-Yi
Lee, Hee Hyung/Kim, Tae Ue/Lee, Jae Dam/Cho, Sung-Woo
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Abstract
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The effects of porcine bone morphogenetic protein (pBMP) and transforming growth factor-0 (TGF-0) on DNA and collagen synthesis were studied in primary cultured fetal rat calvariae cells and cultured osteoblast cells, MC3T3-El. DNA synthesis was slightly but significantly increased 1.2-fold by pBMP and 2.8-fold by TGF-O in fetal rat calvariae cells. In MC3T3-El cells, pBMP and TGF-O increased the rate of DNA synthesis, and the stimulatory action of pBMP was weaker than that of TGF-0. The rate of collagen synthesis was increased 1.4 fold by pBMP and 1.3-fold by TGF-0 in fetal rat calvariae cells. In MOT3-El cells, pBMP and TGF-O increaed the rate of collagen synthesis 1.5-fold and 13-fold, respectively. No synergistic effects of TGF-O and pBMP on collagen synthesis were observed in both cells. Bone matrix gelatin (pBMG), the crude extract obtained during the intermediate step of pBMP isolation, more profoundly stimulated DNA and collagen synthesis than pBMP and TGF-0 did in both types of cells.
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