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KMID : 0578319930030030239
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Molecules and Cells
1993 Volume.3 No. 3 p.239 ~ p.244
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The Region of distribution of Barbiturates in Pure Phospholipid Model Membranes as Studied by Fluresence Quenching
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Kim, Inn Se
Kim, Soon Ho/Kim, Hyung Il/Son, Woo Sung/Kang, Jung Sook/Yun, Il
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Abstract
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The degree of penetration of barbiturates into the model membranes of phosphatidylcholine (SPMVPC) and phosphatidylethanolamine (SPMVPE) extracted from synaptosomal plasma membrane vesicles was determined by employing the fluorescent probe technique. The two fluorescent probes N-octadec)inaphthyl-2-amine-6-sulfonic acid and 12-(9-anthroyl)stearic acid were utilized as probes for the surface and the hydrocarbon interior of the outer monolayer of the SPMVPC and SPMVPE, respectively. The Stern-Volmer equation of fluorescent quenching was modified to calculate the relative distribution. The analysis of preferential quenching of these probes by barbiturates indicates that pentobarbital, hexobarbital, amobarbital and phenobarbital are predominantly distributed on the surface area, while thiopental sodium has an accessibility to the hydrocarbon interior of the outer monolayer of the SPMVPC and SPMVPE. From these results, it is strongly suggested that the more effective penetration into the hydro-carbon interior of the outer monolaver of the membrane lipid bilayer could result in higher general anesthetic activity.
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