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KMID : 0578319930030030251
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Molecules and Cells
1993 Volume.3 No. 3 p.251 ~ p.254
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Analysis of L-myc Restriction Fragment Length Polymorphism in Stomach Cancer by Polymerase Chain Reaction
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Lee, Hee Won
Kim, Dong Wook/Lee, Jae Dam/Lee, Kyoo Hyung/Lee. Sun Sin/Kim, Sang-Hee/Lee, In Chul
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Abstract
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A Polymerase Chain Reaction (PCR) method has been developed for analysis of L-myc EcoRI RFLP (restriction fragment length polymorphism) which originates from the polymorphic EcoRI site in the second intron of the L-myc gene. The patterns of L-myc gene polymorphism were examined using DNAs isolated from cancer tissues and normal tissues of sixty seven patients with stomach cancer. The patients were classified into three genotypes (L-1, I´S, S-S) according to the polymorphic patterns defined by two alleles (L, S). Cancer tissues and normal tissues of the same patients showed the same patterns in the RFLP. Analysis of L-myc RFLP with the clinical pattern of the malignancy showed no significant correlation with metastasis and TNM staging. However, in contrast to other reports, a correlation was found between the RFLP pattern and the size of the tumor (p = 0.005). The preponderance of the L fragment was observed in the larger (>55 mm) sized tumors.
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KEYWORD
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