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KMID : 0578319930030030255
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Molecules and Cells
1993 Volume.3 No. 3 p.255 ~ p.261
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Structural Organization and Sequence Analysis of the Calmodulin Genes from Rice (oryza sativa cv. IR36)
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Choi, Young Ju
Lim, Chae Oh/Shim, Pyung Gyun/Gal, Sang Wan/Bahk, Jeong Dong/An, Gynheung/Cho, Moo Je
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Abstract
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Two genomic clones (RCaM-1 and RCaM-2) encoding a calmodulin (CaM) were isolated from a rice genomic DNA library using a potato CaM cDNA as a probe. RCaM-1 is a 5.5 kb Hindlll restriction fragment and RCaM-2 is a 4.6 kb HindM restriction fragment. The both clones were sequenced and found to contain a complete CaM coding sequences interrupted by a single intron at 25th amino acid. The size of intron in RCaM-1 and RCaM-2 were 1,585 by and 780 bp, respectively. Comparison of nucleotide sequences in the coding region of RCaM-1 with that of RCaM-2 revealed 87% homology, however, 148 deduced amino acid sequences of the two CaM clones were identical except a single amino acid substitution at position 139 (Glu--*Asp). The putative regulatory element in 5´-flanking region of the RCaMI and RCaM-2 shared some common features with their own distinctive structure. RCaM-1 contained consensus TATA box and GC-rich boxes that are found in mammalian promoters, however, RCaM-2 contained distinctive CAAT box and a cyclic AMP regulatory element (CREs) binding site (TGACGTCA). Southern blot hybridization of rice genomic DNA revealed that rice genome contains at least four copies of CaM or CaM-related genes.
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