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KMID : 0578319930030030263
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Molecules and Cells
1993 Volume.3 No. 3 p.263 ~ p.267
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Nucleotide Sequence Analyses of E1 Genes from Korean Hepatitis C Virus Isolates
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Kwag, Eun-Jin
Kim, Jung-Seob/Ann, Hyun-Jong/Min, Mi-Jyung/Yun, Yung-Dae/Moon, Hong-Mo
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Abstract
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We cloned and sequenced hepatitis C virus (HCV) envelope gene [E1, 903-1479 nucleotide (nt), according to Kato´s numbering system, 576 base pairs; Kato et al., 1990] derived from 22 Korean Hepatitis C virus isolates. The E1 genes showed 86-99% and 83-99% homology in the nucleotide sequence and the deduced amino acid sequence respectively. The sequence homology of E1 genes from Korean HCV isolates was compared with Japanese and American HCV envelope genes: 89-93% [in nucleotides (nts)] and 88-95% [in amino acid (AA)] with HCV-J, 59-60/a (in nts) and 59-61% (in AA) with HCLrJ6; 73-75% (in nts) and 73-76% (in AA) with HCV-A. The sequence of HCV22E1 showed more than 11% divergence compared with all other sequences including HCV-J, HC-J6 and HCV-1, indicating that this isolate was a plausible unique Korean type. Twenty two Korean HCV isolates were divided into three subgroups according to their major hydrophilic regions: subgroup 1 had three major hydrophilic regions at amino acid positions of 38-47, 6472 and 104112; subgroup II had three major hydrophilic regions at amino acid positions of 3847, 64-72 and 68-76; subgroup III had three hydrophilic regions at amino acid positions of 3847, 101-109 and 104-112. The number of potential N-linked glycosylation sites ranged from 4 to 6. Among 22 HCV Ell clones, 5 clones had 7 cystein residues while the others had 8 cysteins. The E1 gene products from each subgroup could be mixed and used as a HCV subunit vaccine.
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KEYWORD
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