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KMID : 0578319930030030345
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Molecules and Cells
1993 Volume.3 No. 3 p.345 ~ p.347
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Agrobacterium Mediated Transformation of Nicotiana glauca, a Woody Plant
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Kim, Young Soon
Chae, Soo Cheon/Soh, Woong Young/Suh, Mi Chung/Hong, Choo Bong
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Abstract
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Nicotiana glauca is a fast growing perennial and bears thousands of seeds every year. These characteristics could provide advantages of using N. glauca as a tnodel system for the study of gene expression controls in woody plants. Leaf explants of N. glauca were cocultured with Agrobacterium tumefaciens strain LBA4404 carrying a plasmid pBI121 and in vitro regenerated. Regenerating cells showed strong resistance to kanamycin on the in vitro regeneration medium, and transformants were efficiently selected on the medium containing 300 mg/I of kanamycin. In vitro regenerated N. glauca plants showed specific northern signals for a-glucuronidase transcripts. The seeds obtained from the self-fertilized transformed plants showed a Mendelian pattern of inheritance in kanamycin-resistance. T2 generation plants from the kanamycin-resistant seeds also showed specific northern signals for 0-glucuronidase transcripts.
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