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KMID : 0578319930030040403
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Molecules and Cells
1993 Volume.3 No. 4 p.403 ~ p.406
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Determination of the Nucleotide Sequence Which Affects on the Transcription Inhibition Site of Yeast rRNA Gene
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Kwon, Hyuk Ran
Kim, Jae Hyun/Lee, Chan Hee/Koh, Hung Sun/Choe, Soo Young
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Abstract
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We have previously reported that the Sacchromyces cerevisiae ribosomal gene promoter consists of at least two essential domains, an upstream domain located at the 5´ boundary near position -150 and a core domain around the transcription initiation site at +1 (Choe et al., 1992). We also showed that the yeast ribosomal gene promoter has a critical requirement for binding of protein or protein complex to core and upstream domains to be located at precise positions on the face of the DNA helix (Rho et al., 1993). Here we show the promoter activity and the position of transcription initiation site are highly dependent on the nucleotide sequences neighboring the original +1 site. The substitution of the original +2 nucleotide (T) to C or G shifted the transcription initiation site to -1, but the substitution to A did not. The mutation of the sequences from +13/+17 only affected on the promoter activity, not on the determination of tanscription initiation site. These results suggest that the transcription initiation site of the ribosomal gene promoter requires a typical arrangement of nucleotides around the +1 position.
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