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KMID : 0578319930030040407
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Molecules and Cells
1993 Volume.3 No. 4 p.407 ~ p.416
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Cloning and Overexpression of the Highly Immunogenic Region of HCV Genome from Korea Patients
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Cho, Young Gyu
Yi, Min Kyung/Jang, Kyung Lib/Kim, Chang Min/Sung, Young Chul
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Abstract
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Core, NS3 and NS4 regions of hepatitis C virus (HCN) genome, which are known to be highly immunodominant regions, were obtained from sera of Korean chronic hepatitis patients by a reverse transcriptase-polymerase chain reaction (RT-PCR) method. Comparison of nucleotide sequences and their deduced amino acid sequences of the obtained clones with those of other major HCV isolates showed that there were at least two types of HCV genome in Korea and that our HCV isolates were more closely related to the Japaness isolates, type H and type III, than the U.S. prototype. The core, NS3 and NS4 regions were overexpressed and purified as the form of fusion proteins as well as chimeric polyproteins by using a prokaryotic expression-purification system. These proteins showed the high immunoreactivity with anti-bodies in sera of non-A, non-B hepatitis (NANBH) patients. In particular, a chimeric polyprotein containing core, NS3 and NS4 region, appears to show the highest immunoreactivity, indicating that it may be a good substrate for accurate and/or early detection of HCV infection in Korean patients.
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KEYWORD
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