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KMID : 0578319940040010007
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Molecules and Cells
1994 Volume.4 No. 1 p.7 ~ p.12
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Cloning and Overexpression of Human Immunodeficiency Virus-1 gag and env Derived Epitopes in E. coli
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Chong, Young-Hae
Sohn, Mi Jin/Chang, Ji Eun/Lee, Young Ik
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Abstract
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In order to develop inexpensive and sensitive serodiagnostics, parts of the gag, env genes or gag-env fusion genes of HIV-1 were cloned and expressed in E. coli, using expression vectors containing trp or tac promoter. Immunoreactivities of recombinant proteins were determined using HIV-1 specific antibodies in western blot analysis. Experimental results showed the feasibility of mass production of HIV-1 gag, and env derived proteins which can be used for HIV-1 serodignostics in screening a large number of blood donors to prevent HIV-1 infection.
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KEYWORD
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