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KMID : 0578319940040010013
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Molecules and Cells
1994 Volume.4 No. 1 p.13 ~ p.20
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Phylogenetic Relationship of Cheju Native Horses by Mitochondrial DNA Analysis
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Oh, Moon You
Ko, Mi Hee/Kim, Gi Ok/Kim, Se Jae/Hong, Sung Soo
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Abstract
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The RFLP patterns of Cheju native horse´s mtDNA were analyzed in 52 head from the Cheju (Korea) population using 18 different restriction enzymes. Of the 18 different enzymes used, polymorphism of the cleavage patterns was observed in 4 enzyme (BamHI, EcoRl, Hindnl, and Avail) digestions. By combining the cleavage patterns for each enzyme, 52 head were classified into 9 restriction types. The average number of nucleotide substitution per site (S) was estimated to be 0.0104. The phylogenetic tree constructed _from the 9 restriction types by UPGMA method indicated that mtDNAs of the 52 Cheju native horses are divided into two clusters which diverged approadmately 7.2 X 105 years ago. The comparison of mtDNA cleavage maps of Cheju native horses with those of other species showed that each species contained an average of 55 cleavage sites for 15 enzymes, of which 25 were invariant for all species. Trees constructed according to the parsimony principle, on the basis of 34 phylogenetically informative restriction sites, indicated that Cheju native horse was more closely related to E. przewahkii among species of Equus. We suggest that RFLP analysis of mtDNA is a useful tool for elucidating the origin of Cheju native horses and phylogenetic relationships among species of Equus.
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