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KMID : 0578319940040010051
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Molecules and Cells
1994 Volume.4 No. 1 p.51 ~ p.56
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Mutational Analysis of CRP Binding Siste in the Regulatory Region of glpD and glpE Genes rom E. coli
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Choi, Yong-Lark
Chung, Soo-Yeol/Chung, Chung-Han
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Abstract
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The g1pD and g1pE genes share a common CRP binding site. Expression of the adjacent but divergently transcribed g1pD and g1pE genes is positively regulated by the cAMP-CRP complex. In this study, the transcripts of g1pD and g1pE genes were identified and the positive regulation by cAMP-CRP was also observed at the mRNA level. Several mutations including substitution, deletion, and insertion were introduced into the CRP binding site. We found that several mutants, a I-bp to 3-bp substitution, an altered distance of the block and have the altered motif, affected the expression of g1pD and g1pE at different levels. In deletion mutants, which have a single 5´TGTGA3´ motif, the expression of both genes was activated by the cAMP-CRP complex while its effect is greatly reduced. The binding of the cAMP-CRP was reduced by the deletion and substitution mutants.
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KEYWORD
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