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KMID : 0578319940040010073
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Molecules and Cells
1994 Volume.4 No. 1 p.73 ~ p.78
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Molecular Cloning and Characterization of Schizosaccharomyces pombe Dihydrofolate Reductase Gene
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Choi, See Young
Choe, Won-Chae/Kim, Jiyoung
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Abstract
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The dihydrofolate reductase gene from the fission yeast Schizosaccharomyces pombe was cloned in F_ coli by phenotype of resistance to a folate analog, trimethoprim. The complete nucleotide sequence of a 1372 by DNA fragment containing the dihydrofolate reductase gene of S. pombe is presented. Within this region a single open reading frame of 681 base pairs was found which is capable of encoding a 227 amino acid residue protein with a caculated Mr of 25,325. The amino acid sequence of the S. pombe dihydrofolate reductase (DHFR) derived from the nucleotide sequence has been found to be 49% and 471/6 homologous to human and S. cerevisiae DHFR enzyme, respectively. Several putative TATA elements are found at the 5´ upstream region of the DHFR coding sequence. Northern blot analysis revealed that an mRNA of about 1,500 nucleotides was transcribed from the DHFR gene.
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