|
KMID : 0578319940040010099
|
|
Molecules and Cells
1994 Volume.4 No. 1 p.99 ~ p.109
|
|
|
Nuclear Periphery Localization of RNA1 protein In Saccharomyces cerevisiae
|
|
koh, Sang Seok
Rhee, Young Suk/kang, Hyen Sam
|
|
|
Abstract
|
|
|
|
The yeast rnal-1 is a temperature-sensitive mutation that results in the accumulation of precursor RNAs (pre-RNAs) in the nucleus at the restrictive temperature. We have identified a protein of 48 kDa by immunoblots of yeast extracts using antiserum raised against fusion protein containing regions of RNA1 protein and E. soli lacZ domain. Subeellular fractionation of yeast cells reveals that RNA1 protein is enriched in nuclear fraction. RNA1 protein is localized in the nuclear envelope by indirect immunofluorescence microscopy of whole cell and extraction with Triton X-114 of purified nuclei. It is, however, not an integral membrane protein. In addition, a protein of 39 kDa (p39) which strongly cross-reacts with affinity-purified anti-RNA1 antibody has been identified in the nuclear envelope of mammalian cells. These localization results, together with the pleiotropic effects of rnal-I mutation, may suggest that RNA1 protein may play a role in the nucleocytoplasmic export of RNAs.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|