|
KMID : 0578319940040020183
|
|
Molecules and Cells
1994 Volume.4 No. 2 p.183 ~ p.188
|
|
|
Development of Virus-resistant Potapo by Expression of Phytolacca Antivirl protein
|
|
Moon, Young Ho
Jeon, Hong Seob/Choi, Kyu Whan/Lee, Jong Seob
|
|
|
Abstract
|
|
|
|
Phytolacca antiviral protein (PAP) has been known to reduce viral infectivity. In order to develop potato plants resistant to viruses, PAP cDNA was manipulated under the control of cauliflower mosaic virus 35S promoter (CaMV 35) so as to be expressed in plants. The CaMV 35 S-PAP chimeric gene was transferred into potato cells by mediation of Agrobacterium. Transformed potato cells were regenerated to transgenic potato plants. Southern hybridization of DNAs amplified by polymerase chain reaction from genomic DNAs isolated from transgenic plants indicated that the chimeric gene was integrated into the genome of transgenic plants. Western blot analysis with anti-PAP antibody confirmed the expression of PAP in transgenic plants. In order to investigate whether transgenic plants expressing PAP showed resistance to potato viruses, they were inoculated with potato virus Y (PVY), potato virus X (PVX) and potato leafroll virus (PLRV), and the concentration of particles of each virus was measured on the 45th day by ELISA with antibodies raised against each virus. It was shown that concentrations of PVY, PVX and PLRV in transgenic plants were reduced by 40-fold, 160-fold and 6-fold compared to those in nontransgenic plants, respectively. This suggests that PAP confers the transgenic plants enhanced resistance to all of the viruses tested, and that PAP cDNA may be useful in develoument of transEenic plants resistant to a variety of plant viruse
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|