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KMID : 0578319940040020211
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Molecules and Cells
1994 Volume.4 No. 2 p.211 ~ p.219
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Transgenic Tobacco Plants with Bacillus thuringiensis δ-endotoxin Gene Resistant to korean-Born tobacco Budworms
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Suh, Mi Chung
Hong, Choo Bong/Kim, Sang Seock/Sim, woong Seop
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Abstract
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Insecticidal protein genes, cryM(b) and ciyM(c), of Bacillus thuringiensis subsp. kurstaU HD-1 and HD-73 were expressed in tobacco plants. Two full-lengths and four 3´-portion truncated toxin genes were respectively inserted into a binary vector, pBKS1-1. These six recombinant plasmids amplified in Escherichia co/i were introduced into Agrobacterium tumefackns by direct gene transfer method. After tobacco leaf disks were cocultivated with Agrobacterium carrying these toxin genes, whole plants were regenerated from the transformed leaf disks, and the regenerated transgenic plants (T,) were either self-pollinated or backcrossed. The seeds obtained were germinated on MS media containing kanamycin (300 mg11) and the inheritance of kanamycin- resistant gene introduced to progenies (Tz) was confirmed and followed Mendelian fashion. PCR and Southern blot analyses showed that cryM(b) and cryM(c) toxin genes were stably integrated in the nuclear genomes of the transgenic tobacco plants and inherited to the next generation. Northern blot analysis for the transcripts of neomycin phosphotransferase H gene confirmed that the introduced foreign gene was expressed in the transgenic tobacco plants. Most of the Ti and TZ transgenic tobacco plants showed resistance to lepidopteran insects, tobacco budworms (Hebothis assuka) isolated and maintained in Korea, and the weight increase of larvae feeding on them was inhibited.
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