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KMID : 0578319940040030283
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Molecules and Cells
1994 Volume.4 No. 3 p.283 ~ p.287
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The Effect of ARS-Binding Factor 1 (ABF1) on the Expression of Glycolytic Genes
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Yoo, hae Yong
Jung, So Young/Kim, Young Ho/Kim, Jiyoung/Jung, Guhung/Rho, Hyune Mo
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Abstract
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Autonomously replicating sequence (ARS) binding factor 1 (ABFI) is an abundant yeast regulating protein, capable of binding to ARS and to the upstream region of a number of genes. We examined the effect of ABF1 on the glycolytic genes, phosphoglycerate kinase (PGK) and alcohol dehydrogenase 1 (ADHI) genes. The consensus ABFI binding site was found in the promoter region of these genes. The role of ABFI in regulation of this promoter activity in response to the carbon source was examined by linking 0-galactosidase gene as a reporter. In strain SITY4, ABF1 slightly repressed the expression level of these genes in glucose medium. But following the depletion of glucose and shifting to the glycerol medium, the expression level of these genes was highly elevated by ABFI. In JCA35, an abfl temperature-sensitive mutant strain, ABFI activated these genes regardless of either medium at semipermissive temperature. The activation fold by ABFI in glycerol medium was also higher than that in glucose medium. We therefore suggest that the function of ABFI in the expression of glycolytic genes was affected by the carbon source in the growth medium.
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