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KMID : 0578319940040030295
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Molecules and Cells
1994 Volume.4 No. 3 p.295 ~ p.299
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Cloning and Functional Expression of the ddh Gene Involved in the Novel Pathway of Lysine Biosynthesis frpm Brevibacterium lactofermentum
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Roh, Jung hyeob
Kim, Ok Mi/Park, Dong Chul/Kim, Hyun Jeong/Yun, Hyon Kyung/Kim, Sang Dal/Lee, In-Seon
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Abstract
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The Brevibacterium lactofermentum ddh gene encoding meso-diaminopimelate (meso-DAP)-dehydrogenase (DDH) was cloned by complementation of the Escherichia soli dapD mutation. Sub-cloning experiments and complementation tests indicated that the region for DDH activity was within the ^-2.5 kb XhoI fragment. Southern hybridization analysis confirmed that the cloned DNA fragment originated from B. lactofermentum genomic DNA. The activity staining showed that a specific band of DDH activity was observed in the strains carrying each subclone.
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