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KMID : 0578319940040030343
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Molecules and Cells
1994 Volume.4 No. 3 p.343 ~ p.348
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DNA Strand Break Repair involvement in Hydrogen Peroxide Induced Damage
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Daryl W. Fairbaim
Kim L. O´neill
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Abstract
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DNA repair is an essential function in maintaining proper cellular control. A loss or impairment of repair is associated with many human diseases including malignant diseases. We present here an evaluation of the repair of DNA damage induced by radicals generated from hydrogen peroxide. The comet assay (single cell gel) was used to analyzed damage and its repair in individual cells. DNA damage induced by hydrogen peroxide was heterogeneous, but still displayed dose response relationship with strand break detectability. Rejoining of strand breaks was completed within 60 min, but the appearance of increased breaks consistent with the excision step of excision repair was also observed. Additionally, inhibition of the enzyme poly (ADP-ribose) polymerise by the nicotinamide analog 3-aminobenzamide (0.1 mM and 1.0 mM) was inhibitory of DNA strand rejoining. The study of DNA single strand breaks and repair should provide meaninful insight into the mechanisms of normal and abnormal cellular e-
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