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KMID : 0578319940040030349
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Molecules and Cells
1994 Volume.4 No. 3 p.349 ~ p.354
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Nucleotide Sequence Analysis of the VP2-NS-VP3 Genes of Infections Pancreatic necrosis Virus DRT Strain
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Chung, Hye-Kyung
Lee, Seong-Hun/Lee, Hyung Hoan/Lee, Sae-Sil/Kim, Yong-Sung
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Abstract
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The cDNA of the infectious pancreatic necrosis virus (IPNV) DRT strain was synthesized using the reverse transcriptase (RT)-polymerase chain reaction (PCR) and its cDNA nucleotide sequence was determined. The cDNA nucleotide sequence of the segment A was 3,155 base pairs (bp) in length and con tained two overlapping open reading frames (ORFs). The large ORF was 2,916 by and predicted a polyprotein of a molecular weight of 106,641 daltons, whereas the small ORF was a 444 by and predicted a protein of a molecular weight of 17,369 in IPNV-DRT. The large ORF contains three genes, VP2-NS-VP3, which make a polyprotein. IPNV-DRT and IPNV-Jasper polyproteins are closely related (99/0 amino acid homology). Most of the amino acid mismatches are in VP2 sequences, mainly within the area of the serotype-specific epitope. The small ORF overlapped with the N-terminal part of polyprotein. The 3´ terminal sequence of the DRT strain is longer by 58 by and 51 bp, respectively, than those of the Jasper and NI strains. The DRT strain segment A sequence may not contain a tetranucleotide sequence (CCCC) at the 3´ end. The nucleotide sequence data of the segment A in this paper has been deposited with the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases and has been assigned accession number D26526.
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