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KMID : 0578319940040040439
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Molecules and Cells
1994 Volume.4 No. 4 p.439 ~ p.443
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Development Profiles of DNA-Binding Proteins with a Milk Protein Gene Promoter in Mammary Glands
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Lee, chang Soo
Sang, Byung Chan/Yoo, young Bok/Ryu, Jin Chang/Oka, Takami
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Abstract
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The 5-flanking region of mouse 0-casein gene promoter directs multihormone-dependent transcription. Nuclear proteins from mammary glands of lactating mice formed three major complexes with the 0-casein promoter region from -380 to +7 in a gel mobility shift assay. All binding complexes were specifically competed with one of three highly conserved sequences (called CI, CH and CIM in the different casein promoters. Site CI contains sequences ATGCTAAT having one mismatch of an octamer motif ATGCAAAT as a binding site for transcriptional factors. A synthetic oligonucleotide containing an octamer motif specifically prevented the complex formation with site CI. During the development of mouse mammary glands, three complex-es were detected throughout all stages with an identical electrophoretic mobility, although their binding activities varied depending on developmental stages and changed coordinately. The binding activities were very low at virgin and early-pregnancy and their levels greatly increased at mid-pregnancy and remained elevated through late-pregnancy and lactation, and then fell to a low level 4-days after weaning. Thus, the binding activities reached a maximum level prior to the full activation of the 0-casein gene during development. Three complexes interacting with the three conserved elements were also detected in the kidney and liver.
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KEYWORD
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