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KMID : 0578319940040040449
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Molecules and Cells
1994 Volume.4 No. 4 p.449 ~ p.455
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Genetic Transformation of Streptococcus pneumoniae
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Rhee, Dong-Kwon
Kim, Soo-Hyun/Kim, Seung-Whan/Morrison, Donald
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Abstract
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recP, a recombination gene involved in the genetic transformation of Streptococcus pneumoniae, was cloned in the E. coli transcriptional terminator vectors pKK232-8 and pJDC9, and completely sequenced. Because only a small piece of DNA from the recP region was cloned in these vectors [ghee, D. K., and Morrison, D. A. (1988) J. BaeterioL 170, 630-637], in this study we tried to reconstruct a complete recP gene, and determine the stability both in E colt and S. pneumoniae to test whether the instability was due to strong promoter activity. The whole recP was rearranged in E. soli vector pBR322 and pJDC9, but only a putative promoter of recP was rearranged in the pneumococcus vector pMV158. The E colt clone containing the putative recP promoter region showed very strong promoter activity. The fact that the instability of recP and its strong promoter activity go hand in hand in F. colt, and its stabilization was possible only after deletion of the putative promoter region in the pneumococcus vector suggest that instability of the recP is associated with strong promoter activity.
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KEYWORD
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