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KMID : 0578319940040040481
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Molecules and Cells
1994 Volume.4 No. 4 p.481 ~ p.485
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Protein Kinases in Human cytomegalovirus Infection and Gene Expression
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Lee, Chan Hee
Yon, Joo Hyun/Kang, Kyung Hee
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Abstract
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Infection of human fibroblast cells with human cytomegalovirus (HCMV) resulted in an increase in protein kinase A (PKA) activity measured at 6 h after virus infection. Protein kinase C (PKC) activity increased gradually until 12 h of HCMV infection. The pattern of the increase in PKA or PKC activity demonstrated a close relationship with the increase in the intracellular level of cAMP or Cal+, respectively. The increase in PKA activity may be related to the stimulation of HCMV major immediate early (MIE) gene expression since the treatment of HCMV4nfected cells with stimulators of PKA, including forskolin or bromo-CAMP enhanced the transcription of the MIE gene measured 6 h after HCMV infection. A similar result was obtained when HCMV-infected cells were treated with activators of PKC such as TPA and DOG. On the other hand, verapamil and nifedipine, well-known blockers of Cat+ influx, suppressed the transcription of the M1E gene. Thus our results demonstrate that there is a strong correlation among the intracellular levels of cAMP and Cat+, protein kinase activities, and M1E gene expression in HCMV infection of permissive human fibroblast cells.
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