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KMID : 0578319940040040525
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Molecules and Cells
1994 Volume.4 No. 4 p.525 ~ p.528
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Partial Cloning and Sequencing of the Anaerobiosis-Induced Gene psaA1, in Arabidopsis thaliana Using Reverse Transcription-Random Amplified Polymorphic DNA Technique
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Min, Bokkee
Kim, Eunhee/Kang, Youngwhi/Song, Jae Young/Choi, Soon-Yong/Lee, Se Yong
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Abstract
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Random amplified polymorphic DNA (RAPD) technique is a tool of gene mapping. We used a modified RAPD technique for isolation of stress-induced genes in Arabidopsis thaliana. Total RNA from anoxia-treated Arabidopsis thaliam for 0 min, 30 min and 6 h were purified and first-strand cDNAs were synthesized from the total RNA by reverse transcriptase using random hexamer as a primer. RAPD technique was applied to identify the expression of anoxia-induced genes using arbitrary 10-mer as primers. We have cloned a RT-RAPD product that was produced from anoxia treated Arabidopsis thahana only for 30 min. Through Northern hybridization, we confirmed that the clone was induced by anoxia. Then the cloned RT-RAPD product was sequenced and compared with the databank of GenBank using a BLAST Email Server. The cloned RT-RAPD product was identified as psaAl, the gene of chlorophyll a apoprotein Al.
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