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KMID : 0578319940040050087
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Molecules and Cells
1994 Volume.4 No. 5 p.87 ~ p.92
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RAD4 Gene Required for Nucleotide Excision Repair Is Not UV Inducible and Not Essential for Cell Viability in Saccharomuces cerevisiae
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Kim, Jae Bym
Choi, In Soon/Kim, Dong Sun/Park, Sang Dai
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Abstract
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The RAD4 gene of Saccharomyces cerevisiae is essential for the incisioin step of UV-induced excision repair. We have determined the functional role and the inducibility of this gene. The 1.2-kb Pmll DNA fragment was internally disrupted from the cloned RAD4 gene (pPC 1), and the residual fragment was ligated to produce the integrating plasmid pPC 130. Wild type haploids transformed with this pPC130 showed UV-sensitivity, suggesting that the disrupted RAD4 sequence retains the complementing activity for rad4 mutation. Sporulation of diploids transformed with the UV-sensitive integrants gave rise to four viable haploids per tetrad, indicating that the RAD4 gene is not essential for the viability of haploids under normal growth conditions. The amount of RAD4 gene transcript was´not increased when wild type cells were exposed to UV light. Moreover, no induction of 0-galactosidase activity was observed at various times after UV- irradiation in yeast cells containing the RAD4-lacZ fusion, indicating that the RAD4 gene is not UV inducible.
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