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KMID : 0578319940040050111
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Molecules and Cells
1994 Volume.4 No. 5 p.111 ~ p.117
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Analysis of the Negative Regulatory Site Upstream of Bovine Growth Hormone Gene
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Kim, soo-Mi
Lee, Joon/Roe, Jung-Hye
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Abstract
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We had previously shown that the transcription of bovine growth hormone (bGH) gene in HeLa cells drastically increased when sequences upstream of -240 nucleotide residue were deleted from the bGH promoter [Park et al. (1989) Korean J. Microbiol. 27, 85-911. The location of the putative negative regulatory site (NRS) was determined to be between -335 and -240 nucleotide residues. We cloned and sequenced this 95-bp fragment containing NRS. Sequence homology with several other known negative regulatory sites was found. We further investigated whether there are trans-acting factors in HeLa nuclear extracts which bind specifically to NRS fragment. Gel mobility shift assay revealed that several specific complexes were formed between the NRS fragment and some trans-acting factor(s).
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KEYWORD
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